WebCloning exploits this reaction to efficiently clone PCR products. Once the PCR product is cloned into the pcDNA ™3.4-TOPO® vector and the transformants are analyzed for correct orientation and reading frame, the expression plasmid may be transfected into your cell line of choice. Experiment Outline for Cloning WebThe TOPO TA Cloning Kit for Sequencing can be purchased with a variety of competent cells that deliver different advantages depending upon your needs: General cloning: TOP10 cells (Cat. No. K4500-01, K4500-40) High-efficiency cloning: TOP10 Electrocomp™ Cells (Cat. No. K4560-01, K4560-40)
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WebEach pCR ™8/GW/TOPO ® TA Clonin g® Kit is shipped on dr y ice, and contains 2 or 3 boxes as described in the following table. Upon receipt, store the boxes as Web利用TOPO快速高效地完成PCR克隆. 将PCR扩增的DNA片段 (带有A突出端或平末端) 直接连接至超过40种亚克隆、测序或表达载体内，只需5分钟即可完成——获得多达95%的重组 … datediff ww
CYCV14-CHANGYU pTOPO-TA Kit
WebFeatures of the Zero™ Blunt™ TOPO™ PCR Cloning Kits: Fast and easy— go from PCR to clones in just 3 steps and in as little as 5 … WebTA Cloning ® Kits are also available with PureLink ® Quick Plasmid Miniprep Kit. Product Cat. no. One Shot ® Cells Type of Cells Reactions TOPO ® TA Cloning ® Kit (with pCR ™ 2.1-TOPO ® vector) K4500-01 : TOP10 . chem. competent : 25 . K4500-40 : TOP10 . chem. competent : 50 . K4500-J10 : TOP10 . chem. competent : 10 . K4510-20 : Mach1 ... WebOct 27, 2016 · Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form hydrogen bonds. datediffyear