Fluxor herg assay h9c2
WebFluxxor - Home. Fluxxor is a set of tools to facilitate building JavaScript data layers using the Flux architecture by reifying many of the core Flux concepts. It works particularly well … WebMar 17, 2024 · The FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel activity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format.
Fluxor herg assay h9c2
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Webwater baths appear to act as hERG blockers and can reduce assay performance or cause complete assay failure. 4.1 Predictor™ hERG Tracer Red Store Predictor™ hERG … WebMar 5, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2. Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for …
WebFluxOR Assay Kit (Life Technologies, Carlsbad, CA) following the manufacturers protocol. • Fluorescence signals were elicited by 1mM Tl + and 10 mM K + and detected on FLIPR … WebDec 14, 2015 · Using a commercially available thallium assay kit (FluxOR ... Optimisation and validation of a medium-throughput electrophysiology-based hERG assay using IonWorks HT.
WebThe hERG assay, an in vitro electrophysiologic assay, has become routine since its development in the 1990s. It is one of the recommended studies in the ICH S7B regulatory guideline. This is a voltage clamp assay where a cultured mammalian cell (usually human embryonic kidney, or HEK cell) is transfected with hERG DNA. WebThree K+ Channels, hERG, Kir2.1, and Kv7.2, were differentially expressed in U-2 OS cells using BacMam technology. Following an overnight incubation cells were dye loaded using FluxOR then incubated with compounds or controls. Each of the four compound plates (Tocris) was tested against the three cell lines hERG, Kir2.1, and Kv7.2.
WebApr 8, 2024 · ATP produced by H9c2 cells was assessed by the bioluminescence assay kit (Beyotime Biotechnology, Shanghai, China). In brief, the concentration of ATP was assessed by adding 20 μL of the supernatant into 100 μL of luciferase reagent; the luciferase present in the reagent catalyzes the production of luminescence from ATP and …
WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … bismarck demon wrestling 2021WebThe FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel … darling eye center hhiWebMTT assay was carried out using H9c2 cells to determine a proper dose range for the designed assay. The ECG measurement revealed that intravenous injection of 4-AcO-DET at 2.0 mg/kg and 4-HO-MET at 1.5 mg/kg prolonged the QTc interval in SD rats. In addition, hERG potassium channels in Chinese hamster ovary cells treated test substances were ... bismarck demon footballWebSep 6, 2013 · For ATP depletion assays, H9C2 and RCM’s cells were plated in 96 well plates per the manufacturer’s protocol for 24 hr prior to treatments. For gene expression … bismarck demons boys basketballWebs of hERG inhibitors determined in separate tests. N=4. FLIPR Potassium Kit-4 -2 0 2 0 1 3 Dofetilide Terfenadine Pimozide Log[inhibitor] mM) FluxOr Kit-4 -2 0 2 0.0 0.2 0.4 0.6 0.8 1.0 Dofetilide Terfenadine Pimozide Log[inhibitor] mM Compound) IC 50 (nM) FLIPR Potassium Assay 50) FluxOR Assay Dofetalide 39 53 Pimozide 135 195 Terfenadine … darlingey song lyricsWebThe FluxOR™ Potassium Ion Channel Assay provides a concentrated thallium solution and all necessary buffers, allowing maximum target flexibility and ease of operation in a homogenous format that has been demonstrated for use with cells stably expressing hERG, as well as our BacMam-hERG delivery and expression reagent (Catalog number B10019 ... bismarck demons basketball scheduleWebApr 1, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2.Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for further … darling fabricating