WebMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human Smad2. Background Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. WebTGF-β Induces the Early Phase of STAT3 Phosphorylation Independent of SMADs To formally demonstrate that the TGF-β-induced phosphorylation of STATs is part of the TGFβRI-mediated signaling response, we incubated LX-2 cells with SB431542 (ALK5i), a chemical inhibitor of TGFβRI, before the addition of the ligand.
Immunofluorescence analysis of phospho-SMAD 1/5/8 in
WebThe PathScan ® Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that recognizes endogenous levels of phospho-Smad2 (Ser465/467) and Smad3 (Ser423/425) proteins. A Smad2/3 Mouse Antibody has been coated on the microwells. WebNov 12, 2024 · We investigated TGF-β-responsive Smad2 linker phosphorylations and complex formations over time and across millions of individual cells, and we relate these … eastern washington university maintenance
Macrophage Smad3 Protects the Infarcted Heart ... - Circulation …
WebProduct overview. Phospho-Smad2 (S245/250/255) ELISA Kit (ab279929) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Smad2 protein in your experimental model system, you can verify pathway activation in your cell ... WebJan 31, 2024 · To confirm that the activation of PI3K pathway was indeed POSTN-mediated, we cotransfected SULF2-expressing Hep3B cells with either scrRNA or siPOSTN and showed that the previously observed increase in phospho-AKT and phospho-FAK was aborted when POSTN was suppressed (Fig. 4A–D, last 2 columns). WebFeb 5, 2024 · Transforming growth factor β (TGFβ) is a pluripotent cytokine and regulates a myriad of biological processes. It has been established that TGFβ potently inhibits skeletal muscle differentiation; however, the molecular mechanism is not clearly defined. Previously, we reported that inhibition of the TGFβ canonical pathway by an inhibitory Smad, Smad7, … eastern washington university mba online